Comparative in vitro study of antibacterial activity between colicin E1 or enterocin A1 with classical antibiotics against non-O157 Shiga toxin-producing Escherichia coli

Abstract

The present study was designed to investigate infection by non- O157 Shiga toxin- producing Escherichia coli, which considered highly potentially pathogenic for humans and animals. The samples were previously obtained from children under five years with severe diarrhea, and from dogs suffering from hemorrhagic diarrhea. Isolation and detection of bacteria from diarrheal samples were carried out using traditional and selective media followed by biochemical tests to confirm diagnosis of non-O157 STEC. In vitro study including minimum inhibitory concentration (MIC) was done to compare effect of colicin E1 as an antibacterial agent with most effective antibiotics  by measuring the zone of inhibition on agar  and the results showed that colicin E1 effect demonstrated the highest activity in inhibition of E. coli non-O157 STEC growth than enterocine A1 and enrofloxacin