Physicochemical Properties, Isolation and Identification of Pseudomonas Aeruginosa from the Skin of Some Selected Pond Grown Catfish from Gwagwalada Area Council Abuja

Abstract

This study aimed to investigate the physicochemical characterizations such as the PH, temperature, alkalinity, hardness, biological oxygen demand, turbidity and the isolation of bacteria using the spread plate technique in pond water in Abuja, Nigeria using major pilot farms in the region. The results obtained for physicochemical characterization showed that pH ranged from 6.25 6.73, with the highest pH of 6.73 from Andrew Azazi Barack Pond. The temperature ranged from 25.3-25.8, ammonia (0.26-0.55), alkalinity (46.8-53.6), hardness (19.6-25.7), dissolved oxygen (2.8-5.5), biochemical oxygen demand (2.9-4.4) and the turbidity (34.1-52.2) respectively. The swab sticks were inoculated on the MacConkey agar, Cetrimide agar and Cledagar. The plates were allowed to stand undisturbed for 15 minutes and then the plates were inverted and incubated at 37℃ for 24 hours. After 24 hours, the plates were examined, discrete colonies were picked and streaked on Nutrient agar, and incubated for 24 hours. The plates were observed for growth. Biochemical test such as; catalase, oxidase, citrate, indole and methyl red test were carried out. The presence of blue-green colouration on a Cetrimide agar, Cledagar and Nutrient agar signifies the presence of P. aeruginosa. Finally, the organism was identified as; P. fluorescence strain PF-5 and P. aeruginosa, strain PAO.