Effect of stress environmental conditions on mice fertility

Abstract

The study was conducted in the college of veterinary medicine at the University of Wasit to determine the role of heat stress on mammals. Through the study, 24 mice have been separated into three groups equally: the control group administered distal water and kept in 22±2ºC, stressed mice kept at 32±1ºC and administered distal water for 2 weeks, and thymoquinone groups kept at 32±1ºC and administered thymoquinone 30 mg/kg b.w. for 2 weeks. After the end of the drenched period on the last day of the experiment, mice were not drenched for 24 hours, and ketamine 100 mg/kg and xylazine 10 mg/kg were administered to the scarified mice. 100 mg of testis samples were taken from the testis of all groups and kept at liquid nitrogen for PCR to determine the effects of stress through significant differentiation of HSP70 protein in testis tissue. The experiment showed a highly significant effect of the thymoquinone group (30 mg/kg b.w.) when compared with the stressed group kept at 32±1ºC.