Microscopic, serologic and molecular diagnosis of Giardia lamblia in cattle of Al-Suwaira district, Wasit province (Iraq)

Abstract

This study was conducted from January to December 2023 for 200 samples from different places in Al-Suwayra District in Wasit Governorate, where the microscopical, ELISA test, and PCR and analysis of restriction segment length variation (PCR). The results of the current study used microscopically indicated that 75 cattle were infected with the G. lamblia parasite at a rate of 37.5%. The results showed that in the areas where cattle are located, the highest rate of infection with the Giardia lamblia parasite was recorded in the city (19.5%) and in the Rural (22.5%). concerning the rate of infection with the G. Lamblia parasite, based on the results of the ELISA test, was 20%. The test showed a sensitivity and specificity estimated at 22.6%, and 93.3%. The results of the PCR reaction for the three genes showed total infection rates with the Giardia lamblia parasite amounting to 10.5%. While recorded the infection rate with the Giardia lamblia parasite was based on the results of genes amounting to 4% for the ssu rRNA gene. This study concluded the high prevalence of G. lamblia parasite among the study region suggesting the role of increasing of furthermore studies to investigate the prevalence of parasite in other Iraqi areas