N The Quality of Male Mice (Mus musculus L.) Spermatozoes Which is given Ethanol Leaves Extract of Piper caninum

The Quality of Male Mice (Mus musculus L.) Spermatozoes Which is given Ethanol Leaves Extract of Piper caninum

Abstract

Piper caninum is one of the plant species of Piperaceae family which contain various of secondary metabolism products including alkaloids, flavonoids, steroids and essential oil. The objective of this experiment is to find if mice (M. Musculus L.) that were given the P. caninum ethanol leaf extract would react with spermatozoa quality. For this experiment, we used a Completely Randomized Design (CRD) which consists of 4 handlings, and each handling cconsists of 6 repeats. In this experiment, there are vary handling such as P0 or Control where the mice just only fed and given water and P1 or Handling 1 where the mice are not only fed they are also given  500 mg/kg body weight dosage of P. caninum ethanol leaf extract, P2 or Handling 2 where the mice are given 1000mg/kg body weight dosage of P. caninum ethanol leaf extract. And P3 or Handling 3 where the mice are given 1500mg/kg body weight dosage of P. caninum ethanol leaf extract. The treatment continues for 9 days and the ethanol extract is given to the male mice by gavage method. The observation parameter consists of the number of spermatozoa, spermatozoa viability, and spermatozoa morphology in male mice’s cauda epididymis. ANOVA is used for observation results and if there is any significant difference between handling this experiment and continuing with the LSD test. The result shows that 500, and 1000 mg/kg body weight dosage of P. caninum ethanol extract improved the motility of spermatozoa significantly rather than control. The most effective dosage for spermatozoa motility test is 1000 mg/kg body weight. While it's proven effective in spermatozoa motility tests, the ethanol extract doesn’t effective for mice’s testicle weight, spermatozoa quantity, spermatozoa viability, and spermatozoa morphology.